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Anti-MT-ATP6 antibody (ab102573)抗線粒體MT-ATP6抗體產品信息：
- Product nameAnti-MT-ATP6 antibodySee all MT-ATP6 primary antibodies ...
- Description
Rabbit polyclonal to MT-ATP6
- Tested applicationsICC/IF, WB more details
- Species reactivity
Reacts with: Human
- Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human MT-ATP6.
Read Abcam's proprietary immunogen policy
- Positive control
- This antibody gave a positive signal in Human Skin tissue lysate.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. Concentration 200 µl at 0.1 mg/ml
- PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
Applications
Our Abpromise guarantee covers the use of ab102573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use a concentration of 5 µg/ml. |
| WB | WB: 1/250. Detects a band of approximay 27 kDa (predicted molecular weight: 25 kDa). |
Target
- FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Key component of the proton channel; it may play a direct role in the translocation of protons across the membrane.
- Involvement in diseaseDefects in MT-ATP6 are the cause of neurogenic muscle weakness, ataxia, and retinitis pigmentosa (NARP) [MIM:551500].
Defects in MT-ATP6 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
Defects in MT-ATP6 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
Defects in MT-ATP6 are a cause of mitochondrial infantile bilateral striatal necrosis (MIBSN) [MIM:500003]. Bilateral striatal necrosis is a neurological disorder resembling Leigh syndrome. - Sequence similaritiesBelongs to the ATPase A chain family.
- Cellular localizationMitochondrion inner membrane.
- Information by UniProt
Database links
- Entrez Gene: 4508 Human
- SwissProt: P00846 Human
Alternative names
- ATP synthase subunit a antibody
- ATP6 antibody
- ATP6_HUMAN antibody
see all
Anti-MT-ATP6 antibody images
Western blot - Anti-MT-ATP6 antibody (ab102573)
Anti-MT-ATP6 antibody (ab102573) at 1/250 dilution + Skin (Human) Tissue Lysate - adult normal tissue (ab30166) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 25 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)
Additional bands at : 46 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
Immunocytochemistry/ Immunofluorescence - Anti-MT-ATP6 antibody (ab102573)
ICC/IF image of ab102573 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab102573 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
公司介紹：
Abcam位于英國的劍橋科學園，成立于1998年，專門生產和分銷研究型抗體。主要創立人Jonathan Milner和實驗室同事構思成立一間了解科研人員需要的網上抗體公司，理念是Abcam銷售質量高的抗體，并提供zui全面、誠實、時新的說明書，快速的送貨服務，及高效率的顧客和服務。我們的在線目錄(www.414168.com) 已有差不多100,000種抗體和試劑，并不斷添加，供應予百多個國家。Abcam于2005年11月在倫敦證券交易所上市，在美國馬薩諸塞州、日本東京及香港均設有分公司。
Abcam 一直致力加強產品線，為使研究員更容易找到蛋白質研究試劑產品，在2011并購了美國的MitoSciences公司，加強了免疫分析方面的產品供應；同年也并購了英國的Ascent Scientific 公司，開展了生化試劑的供應。在2012年，Abcam并購了美國的Epitomics 公司，成為一家有的RabMAbs® 供應商。
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